(1). Biolistics (gene-gun) is suitable for

• (a). disarming pathogen vectors
• (b). transformation of plant cells
• (c). constructing recombinant DNA by joining with vectors
• (d). DNA fingerprinting
• (e). None of these

(2). In genetic engineering, the antibiotics are used

• (a). as selectable markers
• (b). to select healthy vectors
• (c). as sequences, from where replication starts
• (d). to keep the cultures free of infection
• (e). None of these

(3). Which one ate following represents a palindromic sequence in DNA?

• (a). 5'-GAATTC-3' 3'-CTTAAG-5'
• (b). 5'-CCAATG-3' 3'-GAATCC-5'
• (c). 5'-CATTAG-3' 3'-GATTAG-5'
• (d). 5'-GATACC-3' 3'-CCTAAG-5'
• (e). None of these

(4). The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

• (a). insertional inactivation of alpha galactosidase in recombinant bacteria.
• (b). inactivation of glycosidase enzyme in recombinant bacteria.
• (c). non-recombinant bacteria containing beta galactosidase.
• (d). insertional inactivation of alpha galactosidase in non-recombinant bacteria.
• (e). None of these

(5). Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

• (a). Algae - Methylase
• (b). Fungi - Chitinase
• (c). Bacteria - Lysozyme
• (d). Plant cells - Cellulase
• (e). None of these

(6). DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

• (a). electrophoresis
• (b). restriction mapping
• (c). centrifugation
• (d). polyrnerase chain reactionx
• (e). None of these

(7). An analysis of chromosomal DNA using the Southern hybridization technique does not use

• (a). electrophoresis
• (b). blotting
• (c). autoradiography
• (d). PCR
• (e). None of these

(8). In vitro clonal propagation in plants is characterized by

• (a). PCR and RAPD
• (b). northern blotting
• (c). electrophoresis and HPLC
• (d). microscopy
• (e). None of these

(9). Which vector can clone only a small fragment of DNA?

• (a). Bacterial artificial chromosome
• (b). Yeast artificial chromosome
• (c). Plasmid
• (d). Cosmid
• (e). None of these

• (a). B - denaturation at a temperature of about 98°C separati the two DNA strands.
• (b). A - denaturation at a temperature of about 50°C.
• (c). C - extension in the presence of heat stable DNA polymerase.
• (d). A - annealing with two sets of primers.
• (e). None of these