(1). Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5'___ GAATTC___ 3' 3'___CTTAAG___ 5'

• (a). Replication completed
• (b). Deletion mutation
• (c). Start codon at the 5' end
• (d). Palindromic sequence of base pairs
• (e). None of these

(2). There is a restriction endonuclease called Eco R I. What does "co" part in it stand for?

• (a). Colon
• (b). Coelom
• (c). Coenzyme
• (d). Cali
• (e). None of these

(3). Agarose extracted from sea weeds is used in

• (a). spectrophotometry
• (b). tissue culture
• (c). PCR
• (d). gel electrophoresis
• (e). None of these

(4). Which one of the following techniques made it possible to genetically engineer living organisms?

• (a). Recombinant DNA techniques
• (b). X-ray diffraction
• (c). Heavier isotope labelling
• (d). Hybridization
• (e). None of these

(5). PCR and restriction fragment length polymorphism are the methods for

• (a). study of enzymes
• (b). genetic transformation
• (c). DNA sequencing
• (d). genetic fingerprinting
• (e). None of these

(6). A singe strand of nucleic acid tagged with a radioactive molecule is called

• (a). vector
• (b). selectable marker
• (c). plasmid
• (d). probe
• (e). None of these

(7). Which one of the following is a case of wrong matching?

• (a). Somatic hybridization - Fusion of two diverse cells.
• (b). Vector DNA -Site for tRNA synthesis.
• (c). Micropropagation in vitro production of plants in large numbers.
• (d). Callus- Unorganised mass of cells produced in tissue, culture.
• (e). None of these

(8). Which one is a true statement regarding DNA polymerase used in PCR?

• (a). It is used to ligate introduced DNA in recipient cells.
• (b). It serves as a selectable marker.
• (c). It is isolated from a virus.
• (d). It remains active at high temperature.
• (e). None of these

(9). For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of

• (a). silver or platinum
• (b). platinum or zinc
• (c). silicon or platinum
• (d). gold or tungsten
• (e). None of these

• (a). 1
• (b). 2
• (c). 3
• (d). 4
• (e). None of these