(1). Restriction endonuclease

• (a). synthesizes DNA
• (b). cuts the DNA molecule randomly
• (c). cuts the DNA molecule at specific sites
• (d). restricts the synthesis of DNA inside the nucleus
• (e). None of these

(2). Gel electrophoresis is used for

• (a). construction of recombinant DNA by joining with cloning vectors
• (b). isolation of DNA molecules
• (c). cutting of DNA into fragments
• (d). separation of DNA fragments according to their size.
• (e). None of these

(3). The linking of antibiotic resistance gene with the plasmid vector became possible with

• (a). DNA polymerase
• (b). exonucleases
• (c). DNA ligase
• (d). endonucleases
• (e). None of these

(4). Polyethylene glycol method is used for

• (a). biodiesel production
• (b). seedless fruit production
• (c). energy production from sewage
• (d). gene transfer without a vector.
• (e). None of these

(5). Which one of the following is used as vector for cloning genes into higher organisms?

• (a). Baculovirus
• (b). Salmonella typhimurium
• (c). Rhizopus nigricans
• (d). Retrovirus.
• (e). None of these

(6). DNA or RNA segment tagged with a radioactive molecule is called

• (a). vector
• (b). probe
• (c). clone
• (d). plasmid
• (e). None of these

(7). Restriction endonucleases are enzymes which

• (a). make cuts at specific positions within the DNA molecule
• (b). recognize a specific nucleotide sequence for binding of DNA ligase
• (c). restrict the action of the enzyme DNA polymerase
• (d). remove nucleotides from the ends of the DNA molecule.
• (e). None of these

(8). Stirred-tank bioreactors have been designed for

• (a). addition of preservatives to the product
• (b). purification of the product
• (c). ensuring anaerobic conditions in the culture vessel
• (d). availability of oxygen throughout the process
• (e). None of these

(9). Which of the following are used in gene cloning?

• (a). Nucleoids
• (b). Lomasomes
• (c). Mesosomes
• (d). Plasmids
• (e). None of these

(10). In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents {i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors I) bacterium II) plasmid III) plasmodium IV) bacteriophage

• (a). I,II,IV
• (b). I only
• (c). I,III
• (d). II,IV
• (e). None of these